I hate you.
I hate how everyone can say everything about all the million freaking things you and your sibling do. How your structure can be simplified down to being between 52-64 Kda, with 4 main domains SH4, Unique, SH2, and SH3, but no one explains what happened to SH1? Did s/he run away? Get cut away in some post-translational modification?And What the fuck do you do? I don't care what you whole extended freaking family does, I only care about you specifically and how do you do it. You ancestor resides in my invertebrate animal model, the ancestors of all your cousins do not - I do not care about them. I am new to learning about you and your culture of kinase activity. I need an introduction to Src for dummies, not an intensive look at me and my family and all the freaking shit we do.
I know you're inactivated by phosphorylation of your c-terminal regulatory tyrosine (Y527) but I also know that phosphorylation of your Y416 in the activation loop is more important. As in your Y537 can be phosphorylated and but you will still be fully active if Y416 becomes pY416. How the fuck is that possible if SH3/SH3 intramolecular interactions are supposed to have you snapped shut and locked?
the only good thing is that your adaptor proteins are relatively easy to understand.
get your shit together please,
that is all
5 days ago