Sunday, November 30, 2008
Seriously, how can you get mad at those eyes?
The point is that if there is trouble to be found, my monkey will find it. Yesterday morning, he found more trouble. On saturday, saturday folks, monkey decided that 6:40am was the perfect time to wake up, happy and full of energy. I don't know about you, but on saturday I prefer waking up after 7am. When it is light out (the sun is up), like a normal human being. Thankfully, I had put up the baby gate and shut the bathroom door before going to bed friday. Thus, when monkey woke up, gave me a kiss and said buh bye before crawling out of my bed, I thought "knock yourself out kiddo". Really how much damage could he do in living room where everything is put away?
I woke up a 7am to my child lying on his tummy playing with my computer sans pajama pants and diaper. Those items of clothing were sitting on the couch.
You have been warned. You have been warned.
Wednesday, November 26, 2008
Tuesday, November 25, 2008
You may have recently received an email from us welcoming you as new member of Mountain Equipment Co-op. We mistakenly sent this message to new and long-standing members alike. Sorry about that. We've identified the cause (classic human error) and we'll do our best to ensure it's not repeated.
Your willingness to receive email from us is important, and we try very hard not abuse the privilege. If you've been an MEC member for several years, hopefully it's not too late to officially welcome you!
We sincerely apologize for our error. If you have any questions, please don't hesitate to contact us at 1.888.847.0770.
I've always knew MEC was a class act
Monday, November 24, 2008
But he will figure out within hours how to overcome the gate that is preventing him for reaching my desk and therefore my computer.(sorry I don't know how to rotate pics in blogger and i'm too lazy to fix it in ilibrary).
He has also fully transitioned into Big Boy bedtime routine. I am not allowed to hold him and neither his dad nor can I lie down with him. We are distinctly told "no AAway".
Oh and he's figured out how to open doors, which means we have to get those stupid doorknob covers otherwise we will have more of this:
It also means we have to put a chain on the front door b/c he knows how to unlock the door.
But he doesn't speak more than a few words.....
Sorry that was a tangent. Anyways, Introverted Post-doc (IPD) does not say good-morning when he walks in and has not said 2 words to me since I've been here. Since he is highly introverted, and my highly extroverted personality can apparently be hard to deal with, I have not taken it personally. Like I said, I am trying not to dislike this individual. IPD annoyed me with his comment about the hiring women and annoys me every morning when he doesn't say good morning. I strongly feel that it is rude to not say good morning especially since he'll say good-morning to the PI as he walks by her office as well as one or two other lab members. However being rude does not = bad person. Rude / ill mannered people can still possess other qualities that would deem them to be nice overall. However, IPD has also demonstrated that he is inconsiderate.
Our lab is focused on dissecting flies in various stages of develoment, from embryos, larvae, adult flies. Which is why we have 2 dissecting microscopes as well as sign up sheets for them. No one ever signs up right now as there seem to only be about 2 of us using them at any given time. Today is not one of those days. Only one of the 2 scopes is in the dark room. When mounting flourescently labelled disc, its important to do that in the dark room. This morning I had to mount labelled discs for use in the afternoon. Prior to my bitching I will fully admit that I should've signed up for the scope in the dark room. I totally take responsiblity for that. If you have NO flexibility in which dissecting scope you can use, SIGN UP in advance. Totally totally take responsibility for that.
This morning I had 3 things to do when I got in. Eat breakfast, load a gel and mount discs. My gel was fucked up by labmate (this is another post) so I ended up having to remake the gel. It took me ~1 hour to get that done and then I went to use the dissecting scope in the dark room. IPD had just sat down when I walked in.
Me - "Hey are you going to be long as I have to mount a couple slides"
IPD - "I just sat down so I will be awhile"
Me - "Ok, well I just need it for 10 mins so when you take a break let me know"
If I was in IPDs position, I would've been like, oh why don't you use it then as I'll be awhile. Why put someone hours behind, when I can wait 10 minutes. IPD did not do that. And yes I know, he has no obligation to either. But here's the thing. There will come a time when he'll need me to do that for him, and I will. Why, because its the nice thing to do. It is what makes labs cohesive.
Plus when he went left the scope to workon something else for a bit and have lunch, he didn't tell me! For fucks sake buddy, when there is a piece of equipment that is in demand, be fucking considerate and let others know they can use it, especially if they have freaking asked you to let them know.!
I am really really trying hard not to dislike him. But he's not helping me.
Sunday, November 23, 2008
I have alot I want to say, but I'm tired. So I wont. Good night
Thursday, November 20, 2008
This week it was the PBS that was fubarred. I had a bunch of wing disc dissections to do yesterday but the larvae were not co-operating. Everything had a weird consistency, the imaginal discs look "off" and I was just not able to dissect out the imaginable discs - they kept disintegrating. First I thought it was b/c I was hungry and hadn't done dissections in about 2 weeks, so just needed to practice on a few. After butchering 10 maggots, I had lunch and hoped I would get my mojo back. Nope something was up. Troubleshooting, perhaps I had put too much triton X in the PBS? After triple checking my calculations (seriously how hard is it to figure out how much 10% triton X to put into 100mL PBS to have a 0.1% triton x solution?), I eliminated that as issue. Maybe it was just a mojo / stars not aligned issue - lets retry in the morning.
This morning, I retried again, 3 maggots later I knew something was up. The only thing that changed between yesterday and 2 weeks ago was that I made up 10x PBS. Granted this was the first time in 3 years I made 10x PBS, but its not that hard to make. Especially Drosophila PBS, its 3 freakin ingrediants - NaHPO4, Na2PO4 & NaCl. Not hard. Only reasonable conclusion (in my head) I fucked up measuring out 3 reagents. I really really should not be here. OMG I'm Dr. Jekyls BF!!!
To see if something was wrong with PBS, I re-checked the pH of 1X PBS solution. PBS for flies can be anywhere from 7.0-7.6 and this solution ended up being over 8.0. WTF?? I had pH'd the 10x to 7.5. Granted I did not recheck the pH when I diluted it to 1X, but I have not experienced ddH20 changing the pH that much before. I must not have used the pH meter correctly (again because it somehow so technically challenging). How could I screw that up? Conclusion - Either the 10x PBS was pH'd incorrectly or I fucked up measuring the reagents so its not buffering properly.
Nothing was left to do, other than to remake the 3 ingredient 10X PBS, diligently checking and rechecking that I have the right reagents, the right weight, everything is completely dissolved before pHing etc. I added enough HCL to make the 10x PBS have a pH of 7.4. Guess what the ph was after diluting to 1x? Over 8! I diluted using ddH20 had been stored in a carboy, which is apparently very alkaline.
Long story short - the carboy h20 is very alkaline for some reason. Always check the ph when diluting down 10x solutions. The carboy H20 was what fucked up my PBS, not me. Whew! I am not blog fodder, maybe I can do this whole PhD thing!
Science never works, its all about the process and troubleshooting. I can not use the results of experiments, or the fact that something does not work as some sort of assessment of my capabilities. I really really need to learn that. You may read me saying that alot. I've read that if you repeat something 5 times before bed it will come true, whadday think?
Tuesday, November 18, 2008
Now that all the parents have stopped laughing at my stupidity, err craziness. Let me enlighten you to what transpired. The boy woke up, he brushed his teeth, changed his clothes and ate breakfast. Then he went stir crazy. He brought me his socks AND his shoes to demand we go out. Its beautiful warm fall day so I took him to kick the ball around, went to the mall to buy him some long-sleeved shirts and then we went for lunch with Aunty JV. He's under the weather, had some fresh air and stimulating morning so he'll sleep when we get home.
1.5 hours later he was finally down for a nap that lasted long enough for me to read the email from my mom with the subject "if we die". Yep my mom gave me her death instructions by email. The monkey is now in the process of calling long distance and destroying my bedroom, but I just need to hide. Funny because if I was any where else I would be missing him. Anyways I'm taking a lesson from Mel and going to starbucks and buying some make up from MAC
On another note, I need to update my blog roll desperately. I have way more blogs in my reader than I do posted. If you should / want to be linked leave me a note / send me an email. I'm hoping to update at some point this week
Monday, November 17, 2008
As a student, I make my own hours. In most lab situations, no one is clocking when you are coming and going, so if you need to leave early to get your child ready for Halloween or take him to the doctor, its OK. The key to having kids while doing graduate studies is having both a supportive and understanding supervisor and labmates. For me, even if my supervisor is supportive, having lab mates sniping about me makes for an uncomfortable environment.
What about the challenges? For one being able to attend those darn 4pm seminars. Due to my personal situation, I am on daycare p/u and d/o duty so its impossible to attend after-hour anything. Hopefully that will be changing, but for now I find the inability to attend the social events and some late seminars makes me feel disconnected from the department. At my oldgrad lab, we used to have fridays@five which was basically hanging out and having beer, it was where friendships were formed, collaborations started and a sense of community established. Once I had the monkey, I could no longer attend as his daycare is off campus.
Not many grad students have kids, so you'll be the odd one out. But as like Dr.Isis, sciencewoman and all the other moms that work, I have learned to work really efficiently. Like today I should get at least 2 articls read, plus my blogs while the monkey sleeps on my lap. With that I must go read some science :)
Friday, November 14, 2008
Thursday, November 13, 2008
Sunday, November 9, 2008
Renee who left a rather racist and full of shit comment on Dr.Isis recent post talking about rising above name calling. I fully agree with Isis on the not resorting to demeaning name calling so despite originally referring to Renee with some denigrating epithets, I have erased them. I however stand by what I said in my comment. FUCK YOU. I guess I have calmed down, or maybe not.
Renee - I hate to be the one to inform you of this, but you come across as hating women because you do. You also appear to very prejudiced against other groups for rather stupid and ignorant reasons. For anyone who thinks the following statement is not indicative of racism is wrong.
Lets deconstruct that statement:
"I don't like most black people not for the color of their skin" - do I really need to explain how not liking a whole group of people based on the color of their skin is um racist. Yah, Yah I know she is saying that is not true, but yet she she is grouping them by their skin color. Based on their skin color, she is assuming they enjoy hip hop and dancing and then using that as the reason she doesn't like them. First its not that she is not friends or doesn't associate with them. She dislikes them. So Renee do you like white people that enjoy hip hop and dancing or is it just the black people who like hip hop and dancing? Why not say I do not like people who enjoy hip hop and dancing? Would that not be the more accurate statement if you weren't first catagorizing on race first?
And how about that assumption that most black people like hip hop and dancing? I agree that that hip hop is heavily intertwined with black culture but that does not automatically mean that all or even most black people enjoy hip hop over and above other musical forms (Lenny Kravitz people, Lenny Kravitz! (not to menion many many others)). Many many black people actually strongly disagree with large aspects of hip hop and some of the messages encompassed within the hip hop culture and are actively involved in opposing it. The assumption you made is akin to assumming that if you're catholic you automatically hate gays and are pro-life. If you're not racist then you're either naive, ignorant or stupid.
How about that last part of your statment "I do have female and black friends...they don't belong to that culture...they belong to my culture" - again why is "your culture" of sci-fi, anime and video games exclusive from hip hop or black culture? - (Note that I separated black and hip hop culture, because although intertwined, they are separate). You are assuming that black people as a whole do not enjoy any of those things. Why can sci-fi, anime and/or video games not belong to that culture?
Lastly, the derogartory tone (again racist / prejudical undertones) with which you refer to those cultures that you hate is rather alarming. Hate is a pretty strong word. You are hating someone for the type of clothes they wear or the music they listen to? You actually judge the seriousness of someone based on whether they like shoes??? You have issues. As I write this post, I am wondering why I have let someone as narrow-minded and idiotic as you keep me up and get me so riled.
Because it people like you, that are in positions of power, who when I walk through a door decide I am a not smart / capable based first on the color of my skin, second on sex and then how I am dressed. And I have not said a word or done a task.
It is because of people like you that Barack Obama did not win by a greater margin.
Friday, November 7, 2008
Well apparently quite difficult when critical reagens like the acrlyamide and temed have gone off! This morning was spent making up small volumes of stacking gels to determine first if it was the temed (since we have like 6 bottles of temed we had to test them all), than if it was the acrylamide. We have determined the acrylamide is off for sure, so we've ordered more in. To make sure I don't fall to far behind, I bummed some acrlyamide off a neighboring lab to make up yet another gel. 3 days later I have an acrylamide gel sitting in the fridge waiting for me on monday morning. Lab work is 90% trouble shooting. Repeat. Repeat. Repeat
Tuesday, November 4, 2008
Monday, November 3, 2008
If Dr.Isis would like to critique scientific journals using a statirical take on it, she totally should because she is rather funny. BUT (you knew their had to be a but) then she is going to have to expect (1) comments that she may few snarky (2) realize that she is NOT modelling the behaviour she herself demanded and (3) understand that just like she doesn't always find her colleagues funny not everyone finds her funny.
I would also like to point out that the authors have made it very very clear that they do not have a problem with individuals / blogs discussing they report, they had an issue with the tone of the original post. Which brings me to CPP, what the hell is the point of name calling on a post that was meant to be a scientific discussio of the findings? If you want to name call, go do it on the original post. Seriously, you are an intelligent individual and by reducing yourself to the standards of my monkey, you are no better then the sick fuck republicans that you hate so much. Dialogue is so important if we are going to move forward on a variety of issues facing us. Can we grow up just a bit?
The biggest issue of all that has come out of this is how the mass media protrays researcher articles / letters. Jansky made a very good point in his comment about how the media choose to pick certain comments and not others. How do we has scientists ensure that our findings are correctly conveyed to the great community?
Sunday, November 2, 2008
I’m not sure how much dancing and “production” one can expect from someone that is >50 years old, I for one was impressed with how fit she was. What I wasn’t impressed with is that she did not start until 9:30pm when the tickets said 7:30pm. Obviously I expected a delayed start, that is only reasonable when you have >50,000 people filing into a stadium. Which is why concerts usually have opening acts. They entertain those front row VIP’s that paid $600.00 for an evening of entertainment. The opening act entertains those fans that got in and are waiting for everyone to finish buying their concert paraphernalia, beer, water, popcorn etc. They keep us going for the 30min – 1 hour that they concert may be delayed. But TWO hours late. Woman I could’ve gone home, showered and most importantly kissed my monkey goodnight. I would not have had to freshen up with some baby wipes in a washroom stall at work (FYI, baby wipes are surprisingly good at freshening one up!).
Once she came on she was as good as could be expected. Any fan knows that Madonna’s not the best singer / dancer but she is a damn good entertainer. I was disappointed with her production and her effort. She barely engaged the crowd, did not have well choreographed dance routines outside of “Vogue” number. There was a lot of jumping around and guitar playing. Yep guitar playing, that annoyed the crap out of me because she ain’t no guitarist. The exception was the hard rock version of Borderline that was wicked! I totally want a bootlegged copy of it.
She took 3 FULL song breaks, as in the dancers were on stage but she was not. Again, she is older so maybe she needed them, I don’t know. When she sang anything before the Ray of Light album, I was totally into it. The more recent stuff was hit or miss for me. I knew some – Music, 4 minutes, Die another Day (she wasn’t on stage for that one) but pretty much nothing from the sticky and sweet album. I definitely was not the only. The crowd went nuts for borderline, material girl, etc but would quiet down for anything that was recent.
Her multimedia effects were very cool and she had a carachi band for La Isla Bonita, You Must Love Me and her new Spanish song. The carachi band was amazing. Overall it was a good show, but not as good as Lenny Kravitz or Pearl Jam for crowd engagement (and obviously those two have amazing music). Janet Jackson’s Janet and Velvet Rope concerts were way better for overall production. Yes I am that old. I will post pics when I get a chance. Now I must go read about epithelial cell development in Drosophila Melanogaster – you know that research that has no public good.